The shows hosts have invariably been female porn stars, who depending on the nature of the call may interact with the caller while performing (non-explicit) sexual acts such as masturbation or playing with sex toys.
In early 2006, Playboy TV cast adult stars Jesse Jane and Kirsten Price in the host roles for the final 14 months of the series' run.
In its final incarnation, the series used a far larger set with striptease poles, a house band and a live studio audience.
Patrick left after the first season as her career ascended but returned in June 2001 as co-host when Flower was pregnant.
In 1998, Ashton and Rone co-starred in a feature-length direct-to-video motion picture entitled Night Calls: The Movie in which the two appeared as themselves.
Playboy Radio, Sirius & XM channel 102, continues to air a version of Night Calls, co-hosted by adult film stars Debi Diamond and Nicki Hunter.