Night of the Serpent (Italian: La notte dei serpenti), also known as Nest of Vipers, is a 1969 Italian spaghetti Western film co-written and directed by Giulio Petroni and starring Luke Askew, Luigi Pistilli, Magda Konopka, Giancarlo Badessi and Chelo Alonso.

After he finds out about the "nest of vipers" and their scheme to get Manuel's inheritance, Hernandez, the corrupt Lieutenant of the Mexican army's garrison in the villa, joins the group of conspirators as their leader and devises a new plan with the intention of framing an innocent man for the future murder of the boy.

Meanwhile the American Luke, an alcoholic ex-gunslinger who is living in Mexico and suffering from a traumatic and unspoken past, is chosen by the revolutionary and small-time bandit General Pancaldo, who is a friend of Hernandez, to be an unwitting pawn in the conspirators' plan.

However, after befriending Manuel and finding out about the vipers' intention of killing both the boy and his adoptive mother María, Luke decides to get back into shape leaving alcohol and facing the conspirators and the Mexican army head on.

The film was shot between Almeria, Cinecittà, and De Paolis Studios in Rome.