His works influenced Emmanuel Tzanes, Elias Moskos, Georgios Klontzas and Theodoros Poulakis.

[5] In 1492, he worked with famous Greek painter Georgios Vlastos and the sculptor Nikolaos Varvarigos.

His works followed the maniera greca tradition but also exhibited qualities of Byzantine paintings and frescos.

Many Cretan painters began to adopt a style mixing Byzantine and Italian techniques.

One work executed by Nikolaos Tzafouris was a triptych Pietà with Saints Francis and Mary Magdalen which can be found at the Ashmolean Museum.

[7] Another example of signed icon from Nikolaos Tzafouris, Christ Bearing the Cross, is in the collection of Metropolitan Museum of Art NYC.