[4] Nikolassee is located on the Bundesstraße 1 road from the Berlin city centre to Potsdam, south of the extended Grunewald forest.

Once part of the Düppel manor, from 1901 onwards the area was developed as a mansion colony (Villenkolonie Nikolassee).

One year later, the settlement received connection to the Berlin-Blankenheim and Wannsee Railway lines with the opening of Berlin-Nikolassee station.

[7] Nikolassee is served by the S-Bahn lines S1,On Friday and Saturday nights only and S7, at the homonymous station.

The locality is also crossed by the motorway A 115 (the former AVUS, exit "Spanischer Allee"), and by the federal highway B1.