The D300S remained the premier Nikon DX camera until the D7100 was released in early 2013.

The D300 has not only a built-in autofocus motor for all Nikon autofocus-lenses, but includes CPU and metering for older Nikon F-mount AI/AI-S lenses and the new generation perspective control lenses (PC-E).

Popular Photography named the Nikon D300 for their official "Camera of the Year 2007" award.

The MB-D10 is an optional accessory battery pack that provides a vertical grip as well as additional shutter release and autofocus buttons, command dials and focus point selection control.

[16] On February 14, 2008, Nikon released a firmware upgrade which resolves an issue where vertical banding can occur when long exposure noise reduction is enabled for shutter speeds of 8 seconds and slower.

Some have added features while others have attempted to correct defects in the original firmware.