Following the outbreak of war with Japan, the squadron was re-formed as a bomber unit in December 1941, at RAF Newmarket.
During this period, it included a significant proportion of aircrews from other Commonwealth countries, many of whom were Royal Australian Air Force (RAAF) personnel.
The squadron suffered significant losses of personnel and aircraft on operations over Burma.
The squadron was formed again on 1 August 1947 at RAF Kabrit in Egypt with the Douglas Dakota for transport duties and was disbanded on 1 May 1948 when it was re-numbered as No.
The squadron was reformed in April 1956 at RAF Dishforth to operate the Scottish Aviation Pioneer in the light transport role and Army support duties, and was disbanded two years later in September 1958 when it was re-designated No.