Initially, it was equipped with a variety of types for training purposes, including Farman HF.20s, Royal Aircraft Factory F.E.2bs, Avro 504s and Sopwith Pups.

[2] The DH.5 had poor altitude performance,[3] and so the squadron extensively practiced low-level flying both prior to and following its move to France on 14 October 1917.

[4] On 20 November, the British launched the Battle of Cambrai, an offensive supported by the use of large number of tanks and No.

During the World War I era, the squadron claimed in excess of 130 victories, and produced eleven aces, among whom were James Anderson Slater, Edmund Tempest, Philip Scott Burge, Thomas Rose, Charles Cudemore, William H. Farrow, Dudley Lloyd-Evans, Edward Dawson Atkinson, and Ronald McClintock.

On 1 March 1936, 64 Squadron reformed at Heliopolis, Egypt, from two flights detached from 6 and 208 Squadrons, both equipped with the Hawker Demon two-seat biplane fighters although it was officially announced that it was based at Henlow, Bedfordshire in order to not give the impression that British air strength in Egypt was being built up.

[9] From May 1938 64 squadron's two-seat Demons were based at RAF Church Fenton, North Yorkshire and the unit was reequipped with Bristol Blenheim Mk I(F) fighters starting in December 1938.

After the outbreak of the Second World War, the squadron was engaged in patrols off the British East Coast and in December 1939 provided fighter defence for the Home Fleet at Scapa Flow from Evanton, Scotland, for a month.

In November 1944 the squadron received the North American Mustang III and flew these for the rest of the war covering daylight raids of the RAF Bomber Command on Germany.

After the end of hostilities the squadron moved to RAF Horsham St Faith and received the Mustang IV in August 1945.

64 Squadron received De Havilland Hornet F.1 twin-engined fighters and moved to RAF Linton-on-Ouse in August of the same year.