SR 326 began at the intersection of Washington and 3rd Streets in downtown Marietta.

SR 326 headed northeast along Washington Street through a residential neighborhood.

Towards the end of its run on Washington Street and adjacent to the Oak Grove Cemetery, the route began climbing a hill.

The route made a sharp hairpin turn and climbed an even steeper hill towards the Emerson Heights neighborhood.

SR 326 would not experience any major changes until the late 1960s, around the time Interstate 77 was completed through the area.