He is the eldest son of Afrobeat pioneer Fela Kuti and a grandchild of political campaigner, women's rights activist and traditional aristocrat Funmilayo Ransome-Kuti.

Femi Anikulapo Kuti was born in London to Fela and Remilekun (Remi) Ransome-Kuti (née Taylor; 1941-2000), and grew up in the former Nigerian capital, Lagos.

[6] He created his own band, Positive Force, in the late 1980s with Dele Sosimi (Gbedu Resurrection), former keyboard player of Fela Anikulapo Kuti.

In 2001, Femi collaborated on his album Fight to Win with a number of US musicians, including Common, Mos Def, and Jaguar Wright.

Femi's track was created in collaboration with hip-hop and R&B artists D'Angelo, Macy Gray, The Soultronics, Nile Rodgers and Roy Hargrove, and all proceeds from the CD were donated to charities dedicated to raising AIDS awareness or fighting the disease.

Femi Kuti's voice is featured in the videogame Grand Theft Auto IV, where he is the host of radio station IF 99 (International Funk 99, described as "playing a great selection of classics from West Africa, the US and elsewhere").

[12] Femi, the son of Afrobeat singer and political activist Fela Kuti, inherited his father's zeal for both music and activism.

He echoed the same sentiments in the 2015 elections by releasing a remix to the song "Politics Na Big Business" featuring Tuface Idiibia and Sound Sultan through his management company, Chocolate City.

[15] On 15 May 2017 Femi Anikulapo Kuti was referred to in the Guinness world records under the catalog of a single note held on a sax in a method called circular breathing.