The museum is composed of a static section tracing the history of the Ontario Regiment, and a "Vehicle Section" with more than 100 operational military vehicles, including jeeps, trucks and tanks, making it the largest collection in North America.

It does this through using artifacts to tell the story of the Ontario Regiment from 1850 to the present day and its collection of historical military vehicles to "bring history to life."

The museum seeks to honour those who came before and those who are currently serving and to make the history accessible to the public in an engaging, relevant, and exciting way.

The museum is a proud member of the Durham Region Community and is often supported by members of the Ontario Regiment, the Ontario Regiment Cadet Corps, Royal Canadian Legion Branches 43 and 637, and 420 Wing (Oshawa)–Royal Canadian Air force Association.

The museum recounts the history through the display of artefacts grouped by type and period - Pre-Confederation, South African War, WW1, WW2, Afghanistan.

These artefacts include uniforms, personal items, weapons (deactivated), medals, pictures, and trophies.

While in this section of the museum, visitors will experience the largest operational, historical military vehicle fleet in North America[citation needed] and teams of volunteers maintaining and restoring them.

The vehicle collection started in 1976 when the then Honorary Colonel of the Ontario Regiment, Norman Wilton, purchased nine Ferret Scout Cars for use as a ceremonial troop.

[citation needed] Some (but not all) of the current inventory are: Since December 2022, a new exhibit features a rare WW2 Howitzer Motor Carriage M7B1, often referred to as the M7 Priest.

The computer game World of Tanks is a partner of the Ontario Regiment RCAC Museum.