[9] In The Organ Trail, players must cross a post-apocalyptic United States in a car in order to reach a sanctuary free of zombies, called Safe Haven.

Players must manage their limited resources, including food, ammunition, medicine, scrap, money, and fuel for their vehicle, in order to complete their journey and keep everyone in their party alive and healthy.

[10] The Organ Trail's popularity led its developers to start a Kickstarter to fund a "director's cut" of the game based on fan feedback and suggestions.

[11][12][13] The Director's Cut features a number of changes to the original game, including a customizable protagonist instead of the above preset characters, "choose-your-own-adventure" style random encounters, boss fights, in-game achievements and online leaderboards.

[2] While the original game has been described as a "1:1 pure parody" of The Oregon Trail, the developers decided The Director's Cut should stand on its own as a title that pushed beyond the source material.