It is served by Amtrak, the national railroad passenger system of the United States, and SunRail, the commuter rail service of Greater Orlando, as well as local and intercity buses.

In Jacksonville connections could be made with trains that originated in either Chicago or Cincinnati:[5] For a period after a strike on the Florida East Coast Railway interrupted service on its coastal route, from 1964 to 1968, the ACL and its successor, the SCL, ran trains making local stops down the Atlantic Coast from Jacksonville to Miami, notably including a stop in Orlando.

In 1993, the Sunset Limited was extended to South Florida, thus providing the first one-seat ride between Orlando and Miami since 1968.

[14] In August 2014, the City of Orlando announced a $2.1 million station restoration project for the historic building.

The project, which was the first major renovation to the facility since 1990, included fixing cracks and leaks in the stucco walls and tile roof, pavement repairs, restroom upgrades, repainting of the building exterior, restoration of the original 1926 wood doors and windows, replacement and relocation of the air conditioning system to the roof of the building (which allowed the original entrance on the side of the building to be reopened), and the installation of a wheelchair ramp from the parking lot to the new station entrance.

It is also the proposed terminus on the planned Orange Blossom Express commuter rail project out of Lake County.