Marie Railroad ("Soo Line") trackage now owned by the Canadian National Railway.

Under successive railroad ownership of the rail line "right-of-way" itself, Operations are currently based in the historic 1916 Soo Line depot, owned by the Osceola Historic Society and leased to the Minnesota Transportation Museum for its rail operations.

The Museum has its headquarters and maintenance base co-located at JSR, which is open to the public for railroad history.

Every fall, 2–3 locomotives and up to 5 cars are ferried to St. Paul for annual servicing before returning to Osceola in late spring.

These are the locomotives that are active or in restoration for service on the Osceola and St. Croix Valley Railway: Information used in this article is provided by Minnesota Transportation Museum, St. Paul, MN and based entirely on material in MTM archives or equipment inventories of the operating division of the Osceola and St. Croix Valley Railway.