The total number of works currently housed in the library is approximately 100,000, and includes books old and new, as well as "periodicals about the history of the health sciences and related areas".

In 1965, both the contents of the library and the interior oak paneling and shelving were moved to their present location within the newly built McIntyre Medical Sciences Building.

Initially most of the windows on the first floor of the Camera were provided with stained-glass coats-of-arms; one for each of the twelve Canadian universities which then had medical faculties.

The fittings and furnishings of the Osler Library were designed by architects Percy Nobbs & George Taylor Hyde.

[6] The stained glass window in the Osler Room depicts the staff and serpent, symbols of healing associated with the Greek god Asclepius, and a held-out book representing the university.