Osteocephalus yasuni is a species of frogs in the family Hylidae.

It is found in the upper Amazon Basin in southern Colombia (Amazonas and Caquetá Departments), Ecuador, and northeastern Peru (Loreto Region) at elevations of 70–250 m (230–820 ft) above sea level.

[1] It is nocturnal, although during some months males can be heard calling during the daytime.

[3] It is locally affected by habitat loss, but there are no major threats to this reasonably abundant and widespread species.

It occurs in a number of protected areas, including its namesake Yasuni National Park.