Ostiglia (Mantovano: Ustìlia) is a comune (municipality) in the Province of Mantua in the Italian region Lombardy, located about 160 km (99 mi) southeast of Milan and about 30 km (19 mi) southeast of Mantua.

In Roman times, Hostilia was a trade hub from Emilia to northern Europe, as it was located on the Via Claudia Augusta Padana.

After the fall of the Western Roman Empire, it was ruled by the Ostrogoths, the Byzantines and, from the 6th century, by the Lombards.

Ostiglia's history was subsequently connected with that of Mantua, losing its strategical importance; its castle was demolished by order of emperor Charles VI in 1717.

Arnoldo Mondadori Editore, the largest publisher in Italy, was founded at Ostiglia in 1907.