A female version of the character named Cleo Cazo / Ratcatcher 2 appears in the DC Extended Universe film The Suicide Squad (2021), portrayed by Daniela Melchior.

[3] After being re-incarcerated, Flannegan escapes his parole hearing using a flute he carved to control rats via a high-pitched frequency, only to be foiled by Dick Grayson disguised as Batman and captured again.

[6] The Ratcatcher possesses the ability to communicate and control an army of rats and in-depth knowledge of Gotham City's sewer system and Blackgate Penitentiary's layout.

Following this, Cazo came to America and used her father's equipment to become a criminal, only to be arrested for armed bank robbery due to authorities considering rats as weapons and incarcerated in Belle Reve Penitentiary.

Sometime later, Cazo and her pet rat Sebastian (vocal effects provided by Dee Bradley Baker) join the eponymous squad to infiltrate and destroy Jötenheim, a Corto Maltesean laboratory containing the alien Starro.