A sister publication, Otokonoko Comic Anthology (オトコの娘コミックアンソロジー), was released from July 11, 2011 to April 15, 2013, and features cross-dressing manga on a given theme varying with each issue.

[1] Issues of the magazine often came bundled with feminine clothing items intended for male readers to wear, which an Oto Nyan spokeswoman described as something to make the otokonoko fantasy more real for readers, and with items chosen based on what characters in the Oto Nyan manga often wear;[2] these included knee socks,[3] a hair pin,[3] a swimsuit,[3] a lingerie set,[4] and a plaid miniskirt.

[11][12] For the main magazine's eleventh issue, it was rebranded Oto Nyan Omega, but put on hiatus after its release on May 25, 2013; the editorial department wrote that they were hoping to be able to bring Oto Nyan back in some form in the future, and asked readers to follow the magazine's website and social media for updates.

In addition to the manga, the magazine includes feature articles,[1][7][19][20] interviews, reviews of anime, video games, and novels with cross-dressing male characters,[21][22] and illustration galleries.

[30][31] Pop culture news site Akiba Blog appreciated Oto Nyan's slightly bigger focus on eroticism compared to Waai!