Ottavio Acquaviva d'Aragona, seniore (1560–1612) was an Italian Roman Catholic cardinal and archbishop.

He studied "belle lettere" and Greek at the University of Perugia and obtained a degree in utroque iure.

Then he went to Rome and entered the Curia, initially as a referendum of the Supreme Court of the Apostolic Signatura.

Pope Gregory XIV raised him to the rank of cardinal of the Catholic Church in the consistory of 6 March 1591 and on 5 April of the same year he received the deaconage of St. George in Velabro.

On 22 April 1602 he opted for the title of Saints John and Paul and on 5 June 1605 for that of Santa Prassede.