He was the eldest son of Henry II, Prince of Anhalt-Aschersleben, by his wife Matilda, daughter of Otto the Child, Duke of Brunswick-Lüneburg.

After the death of his father in 1266, Otto and his younger brother Henry inherited the principality of Anhalt-Aschersleben as co-rulers; but because they were still underage, their mother Matilda assumed the regency of Aschersleben until 1270, when both brothers were declared adults.

Otto continued to rule jointly with his brother until 1283, when Henry (already a provost) renounced his rights.

14 December 1300), daughter of Henry III the White, Duke of Wrocław, and widow of Henry, Lord of Pleissnerland, eldest son of Albert II, Margrave of Meissen.

This biography of a member of a European royal house is a stub.