Otto le Roi (28 November 1878 – 11 October 1916) was a German naturalist of French ancestry.
He worked at the Koenig Museum specializing mainly on birds but also took an interest in the Odonata, amphibia, and molluscs.
After a humanist education at the Apostle Gymnasium in Cologne, he went to the University of Bonn and studied pharmacy.
He passed the state examination in 1904 but decided to follow his interest in zoology and received a doctorate in 1906 for studies on cirripedia.
In 1915 he joined the 11th Jäger Battalion in Marburg as a volunteer and in 1916 he was killed by artillery shelling while serving on a machine gun platoon on the Carpathian Front .