The Owensboro and Nashville Railroad was a 19th-century railway company in western Kentucky in the United States.
It operated daily trains from its terminus in Owensboro to Louisville, Kentucky, and to points east and west.
[citation needed] Its former rights-of-way currently form parts of the class-I CSX Transportation railway.
[citation needed] It was later absorbed by the Louisville and Nashville Railroad.
This United States railway company-related article is a stub.