pSC101 was the first cloning vector, used in 1973 by Herbert Boyer and Stanley Norman Cohen.
Using this plasmid they have demonstrated that a gene from a frog could be transferred into bacterial cells and then expressed by the bacterial cells.
[1] In the early 1970s,[2] Herbert Boyer and Stanley Norman Cohen produced pSC101, the first plasmid vector for cloning purposes.
Soon after successfully cloning two pSC101 plasmids together to create one large plasmid, they published the results describing the experiment, in 1973.
Although the original pSC101 only contained tetracycline resistance and a restriction site for EcoRI, the commercially available pSC101 gained restriction sites for several enzymes, including HindIII, in addition to the EcoRI site.