Many of the works in the museum derive from the cathedral or from other diocesan churches, some suppressed and no longer extant.

This room the first floor, chiefly for use by scholars due to the presence of the chapter library and the diocesan archives, is a room named for St Gregory Barbarigo, bishop of Padua (1664 - 1697), which contains several accounts of the diocese's library and of the cathedral's scriptorium.

Of special interest are documents pertaining to the renaissance library of bishops Iaocopo Zeno and Pietro Barozzi (bishop from 1487-1507), and which include 14th-century illuminated manuscripts, 15th-century incunabula, and pre-16th-century books.

On the north-east side of the salon is the entrance to the chapel of Santa Maria degli Angeli, commissioned by bishop Pietro Barozzi and built in 1495 by architect Lorenzo da Bologna.

On the main altar is found: The oldest liturgical items are found in the Treasure of the cathedral and include: These rooms exhibits works of art from the 14th to the 15th centuries, the most important of which are: