In addition to hardwood forests and white pine, rhododendrons, sedges, grasses, and clubmosses occur in the stream's vicinity.

It flows east-northeast for a few tenths of a mile before entering Hartley Township, Union County.

It then turns southwest for a few tenths of a mile before reaching its confluence with North Branch Buffalo Creek across from Dogback Mountain.

[1] Panther Run joins North Branch Buffalo Creek 9.42 miles (15.16 km) upstream of its mouth.

[3] In a 2008 report, the watershed of Panther Run was ranked eighth amongst sub-watersheds in the Buffalo Creek drainage basin for restoration priority.

[5] A hiking trail in The Hook Natural Area is located along Panther Run and also crosses the stream several times.

[4] Wild trout naturally reproduce in Panther Run from its upper reaches downstream to its mouth.