para-Nitroblebbistatin is a non-phototoxic, photostable myosin inhibitor with low fluorescence.

[1] Its myosin inhibitory properties are very similar to those of blebbistatin.

[citation needed][2][3] para-Nitroblebbistatin has been successfully used in fluorescent imaging experiments involving myosin IIA-GFP expressing live dendritic cells[7] and synaptophysin-pHluorin expressing live neurons.

[8]