Pauline Brunius, née Emma Maria Pauline Lindstedt (10 February 1881 in Stockholm – 30 March 1954 in Stockholm) was a Swedish stage and film actor, screenwriter and film and theatre director.

Jointly with her spouse (John W. Brunius) and colleague, Gösta Ekman (senior), she was the managing director of the theatre Oscarsteatern in 1926–1932.

As a theater manager, she was known for her tough attitude and determination among the gentlemen in the male-dominated entertainment industry, at least in the managerial positions.

As director of the Oscar Theater, she managed to negotiate financial support from Ivar Kreuger, among others.

Among other things, she was criticized for a number of play choices during the Second World War and for a guest performance by Dramaten in Berlin in 1941.