The road runs immediately to the north of a Buffalo and Pittsburgh Railroad line and passes to the south of the Penn State DuBois university campus, gaining a center left-turn lane and heading through commercial areas with some homes.

At this point, PA 255 becomes Bee Line Highway and heads northeast through wooded areas with some commercial development, coming to an interchange with I-80.

PA 255 turns northeast and crosses into Huston Township, becoming Bennetts Valley Highway and continues through more forests with some patches of fields.

[1][2] PA 255 enters Jay Township in Elk County and continues through agricultural and wooded areas with some residences, heading through Scattertown and Force.

The road becomes a four-lane divided highway and heads north through a mix of farmland and woodland with areas of housing.

The route gains a center left-turn lane and runs past more commercial establishments before heading into residential areas on four-lane undivided South St. Marys Street.