PA 616 is a two-lane undivided road that passes through rural areas in southwestern York County, serving the communities of Glen Rock, Seven Valleys, and New Salem.

The route continues to the east of the Old Mill Creek and the rail trail/tourist railroad as it passes through more wooded areas with a few homes.

The road runs through more forests with small areas of homes and fields, continuing into the borough of Glen Rock.

Farther to the northwest, PA 616 winds through a mix of farmland and woodland with a few homes, eventually reaching the community of Seltzville.

From here, the road continues northwest through wooded areas of residences and reaches Hanover Junction, crossing into North Codorus Township.

The route curves north past more farms and woods, intersecting the western terminus of PA 214 near the borough of Seven Valleys.

Upon leaving New Salem, PA 616 becomes Trinity Road and runs through woodland before heading into farmland and crossing the Codorus Creek into West Manchester Township.