He was one of the most prominent students and colleagues of Adrian Willaert during the formative years of the Venetian School, and published several books of madrigals in the 1540s.
This was near the end of the period during which musicians who received their early training in the Netherlands and adjacent areas left their homelands, going to Italy and other locations where demand for composers and singers was greatest.
Perissone became one of Willaert's students, and part of a close group which included Girolamo Parabosco, Baldassare Donato, Jacques Buus, Jacques Du Pont, and Cipriano de Rore; in addition he acquired a reputation as an excellent singer, most likely of high vocal parts.
In 1548 he finally became a member of the chapel of San Marco di Venezia, on an unpaid basis, which was an extraordinary event; presumably this occurred because his singing was highly desired, but there were no formal job openings.
Even though he was a personal friend, he was not significantly influenced by fellow Willaert student Cipriano de Rore, the principal figure in madrigal composition in the 1550s, and whose style marked an extraordinary increase in expressive intensity of the secular vocal form.