In the early 19th century, it was split and partially dispersed, and some of the predella panels were acquired by the Pinacoteca Vaticana.

The work includes a large central panel, depicting the Madonna Enthroned with Child and Angels, which was fully painted by Fra Angelico.

On the left are St. Dominic and St. Nicholas, attributed to Angelico, while on the right are St. John the Baptist and St. Catherine of Alexandria, considered to be mostly by his workshop.

In front of her is a step with vases housing three white and red roses (symbolic of the Virgin's purity and a forecast of Christ's Passion, respectively).

The pavement is decorated with marble tarsia, already used by Fra Angelico in earlier works such as the San Pietro Martire Triptych (1428–1429).