His father, Fileter N. Stephanoff, was a Russian who settled in England and worked painting ceilings and stage scenery, until he committed suicide around 1790; his mother Gertrude Stephanoff (died 7 January 1808) was a flower-painter with Sir Joseph Banks as patron.
[2] Stephanoff became a popular painter of historical and domestic subjects, working both in oils and watercolours.
Stephanoff ceased to work as artist many years before his own death, which occurred at West Hanham, near Bristol, on 15 May 1860.
[2] Stephanoff's works The Trial of Algernon Sidney, Cranmer revoking his Recantation, Poor Relations, and The Reconciliation were engraved; he also furnished designs for The Keepsake and other annuals.
At the Westminster Hall competition in 1843 Stephanoff gained a prize of £100 for a scene from John Milton's Comus.