She was Première dame d'honneur to the queen dowager regent of France, Catherine de' Medici, from 1561 until 1578.
Her great wealth as a widow enabled her to marry in to the royal house by a marriage in 1544 to Charles, Prince of La Roche-sur-Yon.
In 1561, she was appointed Première dame d'honneur by the regent Catherine in succession to Jacqueline de Longwy, who had died in August of that year.
As such, she was the highest ranking woman office holder at court, with responsibility for all the other ladies-in-waiting and the discipline of the household of the queen.
He claimed that Limeuil felt persecuted by the prince, whom she alleged pressured his wife to control and oppress the ladies-in-waiting: "The said princess, at the behest of the said prince her husband, aside from the pains that she gave to all the maids of the Queen, seemed to have a particular animosity towards her and tried to verify whether she was pregnant, often tormenting her in front of the Queen on this matter and others.