However, Phyllobates bicolor is identifiable by the yellow or orange body and black or dark blue forelimbs and hindlegs, hence the name black-legged dart frog.

[2] Phyllobates bicolor are commonly found in tropical forests of the Chocó region of Colombia.

P. bicolor, along with the rest of the Phyllobates species, produce a neurotoxin known as a batrachotoxin that inhibits specific transmembrane channels in cells.

[4] During the breeding period, P. bicolor emits high pitched single notes as a mating call.

[6] In addition to being a way to deter predators, the bright colors of P. bicolor may serve a purpose for sexual selection.

Similar to other amphibians, P. bicolor experiences ontogenetic changes throughout its stages of life from the time they are tadpoles to mature adults.

After emerging from tadpoles, the juveniles frogs are brown or black in color and slowly become more vibrant as they mature.

Due to the presence of the Western Andes Mountains and the San Juan river basin, long distance gene flow in-between populations is virtually impossible.

[10] The frogs cannot naturally produce the toxin, so they derive the nutrients necessary from their diet and surrounding environment.

Because the gate is permanently open and cannot close, there is not an action potential gradient to utilize and signals cannot be received by nerves.

[3] Experiments have been conducted showing that the amount of BTX on the frog's skin is not in a great enough concentration to depolarize the membrane potential, preventing paralysis.

[11] Of the Phyllobates species, the Chocó choose P. bicolor, P. terribilis and P. aurotaenia to extract poison in one of two methods.

In the first method, Chocó Indians rub the tips of the darts against the skin of a live P. terribilis individual.

In the second, Chocó Indians extract the poison from the skin of P. bicolor and P. aurotaenia frogs by skewering them with a special stick and held over a fire.

This process releases the toxins from within the frog's skin and the tips of the darts are rubbed against the secretion.

These frogs are carnivorous despite their tiny size and tend to forage along the floor for ants, beetles, termites, or other rainforest detritus.

[6] Some of these poison dart frogs are solitary and tend to spend their time alone until mating season comes around, however, there have been notable instances of social interaction among organisms.

Typically, a male will call to any nearby females by trilling or buzzing, creating a series of pulsating and repeating high-pitched notes.

Oftentimes, the male will select a suitable location for female oviposition prior to the mate actually depositing her eggs.

Significant drivers of habitat loss include deforestation, cattle grazing, mining, pollution, and illegal crop spraying.

Since the skin is physiologically active in maintaining a regulated electrolyte balance, respiratory gas exchange, and osmotic imbalance, Bd prevents these vital functions and eventually kills the organism.

In South America, Tatamá National Park in Colombia contains and protects hundreds of endangered and endemic species, including P.

Most notably, researchers studying P. bicolor in captivity have noticed a significant reduction of batrachotoxins present on the skin.