[2] P. trinitatis is an arboreal frog with a bright green body, black and yellow eyes, and brown chin and chest.

[4] Frogs are between 50-80mm in length and display sexual dimorphism, with the females being roughly 1.5 times larger in size than their male counterparts.

[2] Researchers have proposed that P. trinitatis stalks its prey due to its slightly considerable size, slow movement, and toe pad shape.

[9] Their research focused on whether the larvae preferred tadpoles of P. trinitatis or Engystomops pustulosus as prey, especially when the density of each species varied.

[11] By probing the glands of P. trinitatis via electrical stimulation, scientists have isolated insulinotropic peptides from the frog's secretions.

[12] These results suggest that P. trinitatis secretes an antimicrobial as part of its immune defence, though the specific mechanism of action is still unknown.

One study found 15 dermaseptin peptides with varying antimicrobial properties and evolutionarily conserved amino acid regions.

Like defence proteins found in other frogs, these peptides exhibited more potency against Gram-positive than Gram-negative bacteria.

Such studies of immunomodulation and insulinotropic activity suggest that these frogs could potentially be used to develop drug templates for anti-inflammatory and type 2 diabetes treatment.

[11] Mating rituals of P. trinitatis transpire within foliage surrounding small bodies of water, encompassing ditches and similar locales.

[16] Male frogs exhibit leg-waving behaviour to communicate non-verbally and to deter potential adversaries.

[10] Researchers have claimed that, unlike other species of frogs, P. trinitatis has no evidence of having hatching gland cells during its development.

[10] One study focused on finding practices to track the frogs for field research and found that neither bobbins[note 1] nor radio tags were suitable.

Radio tags failed to accurately locate the frogs in areas of high altitude or vegetation due to signal interference.