He learnt the theatre in Auxerre before going to Paris in the early 1950s, where he met Roger Blin and Jean-Louis Barrault.
Among the interpreters of Louki's more than 200 chansons (besides himself) were Lucette Raillat, Catherine Sauvage, Francesca Solleville, Isabelle Aubret, Les Frères Jacques, Juliette Gréco, Jean Ferrat, Philippe Clay, Colette Renard, Annie Cordy and Georges Brassens.
He toured with the latter and wrote a book of recollections entitled Avec Brassens (éditions Christian Pirot, 1999, ISBN 2-86808-129-0).
Pierre Louki also appeared as stage author and actor and broadcast on France-Culture, while on television he took part in programmes of Jean-Christophe Averty.
He wrote several books for children and his memoirs are Quelques confidences (éditions Christian Pirot, septembre 2006).