[citation needed] At the time of Portuguese colonization in Brazil, native Brazilian tribes of different ethnicities lived in the region of today's city of São Paulo.
Caiubi,[3] brother of Piquerobi and Tibiriçá, was chief of the village of Jurubatuba[4] (today Santo Amaro, a suburb of Greater São Paulo city).
At the beginning of colonization in São Vicente, the Portuguese explorers João Ramalho and António Rodrigues, married native Indians; João Ramalho with Bartira, the daughter of Tibiriçá and Antonio Rodrigues with Antónia Ussú (born Assú-Piquerobi), daughter of chief Piquerobi.
Although Piquerobi had a Portuguese son-in-law, he disagreed with Tibiriçá, who had become a Christian and allowed pioneers and Jesuits to settle in the region, who began to enslave the Indians and interfere in religious rituals.
In 1534, in an attempt to expel the colonists, Piquerobi joined the Spaniards, along with some Portuguese, to attack the Vila de São Vicente, looting and destroying it, in a conflict that lasted two years and became known as the Iguape War.