The line remains in service today and is owned by CSX Transportation, which it operates as their Plant City Subdivision.

The Seaboard Air Line bought the Plant City, Arcadia, and Gulf Railroad in 1905 and rebuilt it.

From 1926 to 1952, the Seaboard would designate the former Plant City, Arcadia, and Gulf Railroad as the northernmost segment of their Fort Myers Subdivision (which would overlap the Valrico Subdivision from Welcome Junction a short distance to Edison Junction, where Seaboard's track continued south and connected to Fort Myers at the time).

[4] By 1940, the line would be used by Seaboard's local passenger trains to Boca Grande as well as their Cross Florida Limited (which ran from Tampa to Miami).

[4] After abandonment of Seaboard's track to Fort Myers, the line was considered a spur of the Valrico Subdivision.