The main line ran from Richmond, Virginia to Tampa, Florida, a distance of over 800 miles.

Along its route it passed through Petersburg, Raleigh, Columbia, Savannah, Jacksonville, and Ocala, Florida.

This first talking hot box detector was installed on the main line in Riceboro, Georgia.

[8] The two main lines crossed each other in Chester, Savannah, Jacksonville, and Plant City.

CSX Corporation initially operated the Chessie and SCLI Systems separately, however in 1982 they began the process of consolidating the railroads of both holding companies.

Some of the supports that once held the S-Line's bridge over the Appomattox River are still standing on the northwest side of Petersburg.

The states are doing preliminary work to rebuild the line for high-speed passenger service as part of the Southeast High Speed Rail Corridor.

From Savannah to the Ogeechee River in Richmond Hill, Georgia, the line was abandoned and consolidated with the A-Line shortly after the 1967 merger.

[14] The S-Line was severed as a through route between Savannah and Jacksonville in 1986 when track was abandoned between Riceboro and Bladen, Georgia.

Both the First Coast Railroad and the Riceboro Southern Railway took over their respective sections of the S-Line in the mid 2000s and are both owned by Genesee & Wyoming.