Alongside detachments from the Einsatzgruppen of the SD, it perpetrated mass murder in the Holocaust and was responsible for large-scale crimes against humanity targeting civilian populations.

[1] Twenty-three Order Police battalions were slated to take part in the 1941 invasion of the Soviet Union, Operation Barbarossa.

Two battalions were assigned to support the Einsatzgruppen, the mobile death squads of the SS, and the Organisation Todt, the military construction group.

When the regiment crossed the German-Soviet border, it came under the control of Friedrich Jeckeln, the Higher SS and Police Leader (HSS-PF) for Army Group South in Ukraine.

Shortly thereafter, Police Battalion 320 reported the shooting of twenty-two hundred Jews at another location north-east of Kamianets-Podilskyi.