Alongside detachments from the Einsatzgruppen and the 1st SS Infantry Brigade, it perpetrated mass murder in the Holocaust and was responsible for large-scale crimes against humanity targeting civilian populations.

Two battalions were assigned to support the Einsatzgruppen, the mobile death squads of the SS, and Organisation Todt, the military construction group.

[2] The goals of the police battalions were to secure the rear by eliminating the remnants of the enemy forces, guarding the prisoners of war, and protecting the lines of communications and captured industrial facilities.

[5] When the regiment crossed the German-Soviet border, it came under the control of Friedrich Jeckeln, the Higher SS and Police Leader (HSS-PF) for Army Group South in Ukraine.

[5] Upon formation, while still in occupied Poland, Police Battalion 314 participated in the round-ups of Polish civilians for deportation to slave labour to Germany.

[7] During the summer months, the battalion took part in joint actions with the 1st SS Infantry Brigade of the Waffen-SS, both providing support and undertaking independent killings.

[9] The killing activities of both the Einsatzgruppen detachments and the Police Regiment South slowed down the farther the Wehrmacht advanced, as more Jews were able to escape east and the density of the pre-war Jewish population was lower in Eastern Ukraine.

[11] While the activities of the Police Regiment South, the Einsatzgruppen detachment and the 1st SS Brigade, progressed, the reports by the murder squads were being intercepted and decoded by MI6, the British intelligence service.

As part of Ultra, British signals intelligence program, the codebreaking facilities at Bletchley Park decoded and analysed the messages.