At the age of eight, in Vilnius, she performed Johann Sebastian Bach's Concerto in D minor with the Lithuanian Chamber Orchestra conducted by Saulius Sondeckis.

In 1987, when Osetinskaya was eleven years old, she made her debut in the Great Hall of the Moscow Conservatory [ru], where she played Mozart's Concerto No.

Osetinskaya continued her musical education at a lyceum school in St. Petersburg, where Marina Wolf was her teacher.

Osetinskaya has performed with such conductors as Tugan Sokhiev, Alexander Sladkovsky, Vasily Sinaisky, Teodor Currentzis, Saulius Sondetskis, Thomas Sanderling, and Dmitry Sitkovetsky.

The pianist's recordings have been released on many labels – Sony Music, Naxos, Bel Air, Quartz, etc.

Osetinskaya created and headed the "Polina Osetinskaya Professional Health Support Center for Musicians",[6] which helps musicians and people of creative professions in solving problems related to the peculiarities of the profession, such as overplayed hands, muscle cramps, fear of going on stage, vegetative stress and many others.