Port Vale F.C. league record by opponent

In the club's early history, there was no league football, so matches were arranged on an occasional basis, supplemented by cup competitions organised at both local and national level.

[3] At this stage, North Staffordshire Church League champions Cobridge Church sought permission from the Football Association to change the club's name to Port Vale and bought the old club's ground.

[6] Port Vale's first team has competed in many nationally contested leagues, and their record against each club in those competitions is summarised below.

The opening match of the 1888–89 Combination season pitted them against Birmingham St George's,[7] their first Football League match was against Small Heath,[8] and they met their 200th and most recent different league opponent, Sutton United, for the first time in the 2021–22 season.

Barnsley have beaten Port Vale 47 times in the league, more than any other team, whilst Port Vale have likewise recorded more league victories against Barnsley than against any other club, having beaten them 43 times out of 106 attempts.