Portrait of Elisabetta Gonzaga is an oil on wood painting attributed to the Italian Renaissance artist Raphael, completed c. 1504–1505, and today housed in the Uffizi Gallery, Florence.

The painting was likely part of the Ducal collection of Urbino, brought to Florence in 1635 as Vittoria della Rovere's dowry.

It is mentioned with certainty for the first time in 1773, when it was transferred from Palazzo Pitti to the Grand Ducal wardrobe of the Uffizi.

Details include the black dress with applied trim in a patchwork pattern, and the scorpion-like ferronière on the woman's forehead.

Her hairdo includes the coazzone, a long plait which is present also in a medal of her now at the British Museum.