[2] She studied in Vasant Vihar High School and graduated with a Bachelor of Mass Media from V. G. Vaze College of Arts, Science and Commerce in Mulund.

[6][7] Koli began her career as an intern at Fever 104 FM radio station in Mumbai, and launched a YouTube channel.

[18] In 2023, she headlined a Zee Theatre teleplay called Yeh Shaadi Nahi Ho Sakti directed by Akarsh Khurana.

[19][20] She featured in Anu Menon's mystery film Neeyat, alongside Vidya Balan, Ram Kapoor, Rahul Bose, Mita Vasishth, Neeraj Kabi, Shahana Goswami, Amrita Puri, Dipannita Sharma and Shashank Arora.

[22][12][23][24] Koli was a part of a 9-episode Audible series set in Sydney called Desi Down Under, along with Adarsh Gourav and Taaruk Raina.

[38][39] In 2018, Prajakta was invited to the United Nations headquarters on the occasion of International Day of Tolerance, as an ambassador for YouTube's global initiative, Creators for Change program.

[45][41] Prajakta represented India at The Call To Unite event which started on 1 May 2020 and featured Oprah Winfrey, Julia Roberts, Eva Longoria, Deepak Chopra, Alanis Morissette, Quincy Jones and Mandy Moore among others.

[49][50] On 30 October 2021, Koli made a contribution to TED's campaign called 'Countdown,' which focused on addressing the issue of climate change.

[51] She collaborated with personalities including Al Gore, Chris Hemsworth, Priyanka Chopra, Mark Ruffalo, and Don Cheadle, in discussions and initiatives centered around climate change awareness.