This is a list of winners and nominees of the Primetime Emmy Award for Outstanding Guest Actress in a Comedy Series.

[1] Prior to 1989, the category was not gender-specific, and, thus, was called Outstanding Guest Performer in a Comedy Series.

It is given in honor to an actress who has delivered an outstanding performance in a guest-starring role in a television comedy series.

The most awarded actress is Cloris Leachman, with 3 wins, followed by Tina Fey, Colleen Dewhurst, Kathryn Joosten, Jean Smart, Tracey Ullman, Betty White, and Maya Rudolph, with 2 wins.

Beginning with the 77th Primetime Emmy Awards, performers are no longer eligible in guest acting categories if they were previously nominated for a lead or supporting award for playing the same character role in the same series.