Eleonore Juliane was a daughter of Albert II, Margrave of Brandenburg-Ansbach (1620–1667) and his second wife Countess Sophie Margarete (1634–1664), daughter of Joachim Ernst, Count of Oettingen-Oettingen.

On 31 October 1682, in Ansbach, she married Frederick Charles, Duke of Württemberg-Winnental, with a commemorative coin struck for the wedding[1] At the wedding Eleonore's brother John Frederick met the composer Johann Sigismund Kusser, who later worked at Ansbach.

[2] After her husband's death, Eleonore moved to Ansbach in 1710 to support her youngest daughter.

Eleonore came into personal contact with August Hermann Francke during this time and also wrote songs.

Philipp Friedrich von Geismar acted as her advisor and steward.