James Giddings became director in 1934, Timothy Cheney in 1940, J. Merrill Knapp in 1941, Russell Ames Cook in 1943, J. Merrill Knapp again in 1946, Elliot Forbes in 1952, Carl Weinrich in 1953, Walter L. Nollner in 1958, William Trego in 1992, Richard Tang Yuk in 1994, Robert Isaacs in 2009, and Gabriel Crouch since 2010.

They gave the American Premiere of Stravinsky's Oedipus Rex with Leopold Stokowski and the Philadelphia Orchestra in 1931; performances of Schoenberg's Gurrelieder and Wagner's Parsifal in 1932 and 1933; Bach's Mass in B Minor at the Metropolitan Opera House in 1935; and with the Vassar College Choir, gave the first United States performance of Jean Philippe Rameau's Castor et Pollux in 1937.

The Glee Club continued to sing with women's choral groups from Vassar, Bryn Mawr, Wellesley, Mount Holyoke, and Smith Colleges until a mixed Princeton chorus was formed after the advent of coeducation.

The Glee Club has continued their tradition of overseas concert tours (most recently traveling to Buenos Aires, Paris, Leipzig, Prague, South Africa, Spain, and Mexico) and expanded the repertoire to include more works of the twentieth and twenty-first centuries.

Recent masterworks performed include Orff's Carmina Burana, Mendelssohn's Elijah, Bach's St. Matthew Passion and Mass in B minor, Mozart's Requiem, and Honneger's Le Roi David.