He took part in the Rajput alliance against the Mughal emperor Babur, fighting against the latter in the Battle of Khanwa alongside Rana Sanga of Mewar in 1527.

Three of Prithviraj's sons successively followed him as ruler of Amber, with many of his descendants also populating the kingdom's highest aristocracy in subsequent centuries.

[1] With his rule, Amber, which had been experiencing a long period of stagnation, was revitalised, entering an era of extensive political activity.

[1] In March 1527, he, alongside his son Jagmal and a number of relations, fought under Rana Sanga of Mewar in the fatal Battle of Khanwa against the Mughal emperor Babur.

Like the latter, who was poisoned by his nobles in order to avoid further conflict with Babur, historian V.S Bhatnagar suggests that Prithviraj's death may also have been unnatural, noting that his successors later readily offered their allegiance to the Mughals.