His paternal grandparents were Raja Prithviraj Singh I and his wife Bala Bai, a daughter of Rao Lunkaran of Bikaner.
Ratan Singh's paternal uncles, Sanga and Bharmal, also turned against him and attempted to launch a conspiracy, though this proved less successful.
Already the ruler of much of Rajputana, Suri was a formidable threat who had already forced the submission of the likes of Maharana Udai Singh II of Mewar.
Perhaps taking cue from the latter, Ratan Singh likewise surrendered and accepted the suzerainty of the Afghan ruler.
These individuals instigated his younger brother, Askaran, to make a bid for the crown and on 15 May 1548, Ratan Singh was fatally poisoned.